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Transformative discoveries in genome and cellular integrity

CPES Facility

About the Facility.


The Cell and Protein Engineering and Screening Facility was set up to provide technical services and advice that will promote and advance research projects within the Institute.


The CPES currently provides the following services:

  • DNA cloning into mammalian and bacterial expression vectors
  • Stable cell line generation
  • Knockout cell line generation using CRISPR/Cas9 technology
  • Assistance with setting up CRISPR screens, including library preparation
  • Cell survival assays
  • Assist with yeast genetic screens/DNA preparation
  • Generation of recombinant proteins in bacteria for antibody generation
  • Help with individual projects, including candidate protein characterisation

Expertise:

  • Molecular biology
  • Tissue culture (stable cell lines, knockout cell lines, survival assays, siRNA and CRISPR-based screens)
  • Recombinant protein production
  • Yeast DNA extraction and screening

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